Elicitor에 의한 baccatin III의 세포내 생합성 조절연구

Elicitors for the Regulation of baccatin III Biosynthesis in Plant Cell Culture System

The yeast extract, coconut water, safflower seed oil, arachidonic acid, linolenic acid, jasmonic acid and methyl jasmonate were added to Gamborg's B5 medium. The changes on productivity of baccatin III were estimated every 30 minutes and the results were compared using the selected high yielding cell culture system of Taxus cuspidata. In most cases, the peaks of baccatin III productivity occurred at 90-120 min after addition of elicitors. Among the compound elicitors, safflower seed oil showed the highest productivity of baccatin III. Also arachidonic acid and linolenic acid increased the baccatin III production.