과체 성분의 간질환 모델에서의 효과

Effects of the Constituents of Melonis Pedicellus in the Animal Models of Hepatic Diseases

In order to investigate the hepatoprotective constituents, Melonis Pedicellus was systematically extracted and fractionated with n-hexane, ethyl acetate, butanol and Water. Treatment of rats with ethyl acetate fraction reduced hepatic injuries induced by alpha-naphthylisothiocyanate or D-galactosamine, whereas the components in water fraction showed protective effect only against D-galactosamine-induced hepatitis in rats. Two cucurbitacins and three sterols were isolated from ethylacetate fraction and their chemical structures were identified as cucurbitacin B, isocucurbitacin B, alpha-spinasterol, stigmast-7-en-3-ol and stigmast-7-en-3-ol-0-beta-D-glucopyranoside. Cucurbitacin B at the dose of 1mg/kg (p.o.) significantly increased in bile flow in rats with ANIT-induced cholestasis. Isocucurbitacin B at 5mg/kg (p.o.) showed significant protective effects against ANIT-induced cholestasis. These results showed that cucurbitacin B and isocucurbitacin B from Melonis Pedicellus may have hepatoprotective effect in rats with experimental cholestasis.