RBL 2H3 비만세포에서 히스타민 유리와 칼슘과의 관계

Relationship Between Histamine Release and Ca2+ Mobilization in RBL 2H3 Mast Cells

To investigate the relation between extracellular Ca2+ and histamine release, we observed agonist-induced histamine release from RBL 2H3 mast cells in the presence or absence of extracellular Ca2+ concentration. Histamine release induced by melittin and thapsigargin were greater in the presence of extracellular Ca2+ than in the absence of extracellular Ca2+. Econazole-induced histamine release had nothing to do with extracellular Ca2+, whereas arachidonic acid-induced histamine release increased in the absence of extracellular Ca2+. Calmodulin antagonists did not affect melittin-induced histamine release but they may potentiate arachidonic acid-induce histamine release. These data suggest that arachidonic acid-induced histamine release may be mediated via Ca2+ -independent pathway and may be potentiated by the block of Ca2+ -dependent pathway.