Ethaverine 화합물이 P815 세포중의 Serotonion 함량에 미치는 영향

Inhivitory Effects of Ethaverine on Serotonin Content in Muine Mastocytoma P815 cells

The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5~10μM decreased serotonin content in a concentration-dependent manner. The IC50 value of ethaverine was 6.1μM. Ethaverine at concentrations up to 20μM was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5μM), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.