백출 지상부의 항산화 성분

Anti-oxidative Compounds from The Aerial Parts of Atractylodes macrocephala Koidzumi

Atractylodes macrocephala has been used for renal anorexia, gastroenteritis, cold, dyspepsia in Korean folk medicine. Specially aerial parts has been eaten as edible mountain herbs. In order to investigate the efficacy of antioxidant activity, the activity guided fractionation and isolation of physiologically active substance were performed. For the investigation of the active components from Atractylodes macrocephala MeOH extracts of aerial parts of Atractylodes macrocephala Koidzumi L. were suspended with H2O, partitioned by CHCl3. In order to investigate the efficacy of antioxidative activity, the activity guided fractionation and isolation of physiologically active substance were performed. CHCl3,, H2O, 30% MeOH, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH method. It was revealed that 30% MeOH and 60% MeOH fractions have significantly antioxidant activity. From 30% MeOH and 60% MeOH fraction, six flavonoids (7-methoxy-pinocembrin-7-O-β-D-glucopyranoside, apigenin-8-C-β-D-glucopyranoside, 4'-caffeoyl-luteolin-6-glucopyranoside, luteolin-6-C-β-D-glucopyranoside, apigenin-6-C-β-D-glucopyranoside, luteolin) and four phenylpropanoids (3-feruloylquinic acid 4,5-di-O-cafeoyquinic acid, feruloyl acid, 3,5-di-O-caffeoylquinic acid) were isolated. To investigate the antioxidant activities of each compounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipoprotein (LDL) with TBARS assay. Six compounds (Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅸ, Ⅹ) which hae antioxidant factor showed significant activities.