흰쥐의 일차배양 간세포에서 Daidzin, Daidzein, Genistein 및 Puerarin의 간 보호 활성 평가

Hepatoprotective Activities of Daidzin, Daidzein, Genistein and Puerarin in Primary Cultured Rat Hepatocytes

The aim of this study was to investigate the protective activities of daidzin, daidzein, genistein or puerarin, active isoflavonoids of Puerariae Radix, on the hepatocyte injury induced by carbon tetrachloride (CCl4, 10μM), tert-butyl hydroperoxide (TBH, 0.5mM) and D-galactosamine (GalN, 30mM). Primary cultures of rat hepatocytes (18 hr cultured) were treated with CCl4, TBH or GalN and various concentrations (0.1, 1, 10 and 100μM) of daidzin, daidzein, genistein or puerarin. CCl4 significantly increased the levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The increase in LDH level was attenuated by daidzein, genistein and puerari. Puerarin alse inhibited the increase inAST level induced by CCl4. The increases in LDH and AST levels induced by TBH were significantly attenuated by daidzin and genistein treatments. GalN markedly increased the levels of LDH, ALT and AST. These increases were significantly attenuated by daidzein. Daidzin also inhibited the increases in LDH and AST levels induced by GalN. The increases in LDH and ALT levels were attenuated by genistein and puerarin, respectively. These results suggest that daidzin and daidzein possess hepatoprotective activities.