설치류에서 Ginsenoside Rg1의 항스트레스 활성

Anti-stress Activities Ginsenoside Rg1 in Rodents

Ginsenoside Rg1 (Rg1), the pharmacologically active constituent of ginseng (Panax ginseng C.A.Meyer), has a variety of biological activities. The present study was undertaken to evaluate a possibility of Rg1 whether it can be used in treatment or prevention of stress disorders. Animals were stressed by immobilization for 2 hours or electroshocks for 20 minutes. The normal group was not exposed to any stress. Rg1 was subcutaneously injected as dosages of 5 and 10 mg/kg and red ginseng (RG) was orally administered 200 mg/kg as the positive control. Animals were given supplements for 5 days without stress, and then were given supplements for 5 days with stress. We recorded stress-related behavioral changes of experimental animals using the Etho-vision system. Weight of adrenal gland and levels of corticosterone in plasma were measured and stress related behaviors (smelling, grooming, face washing, rearing) were observed. Rg1 didn't make significant behavioral changes in total open field and elevated plus maze test. Rg1 did not influence on behavioral changes induced by electroshock stress. Whereas, 10 mg/kg of Rg1 alleviated the increment of the freezing and face washing time and the decrement of the smelling and rearing time induced by restraint stress. The administration of Rg1 10 mg/kg has significantly increased the endurance time on rotating rod and swimming pool tests compared to the control group. These results indicate that Rg1 can alleviate the damage induced by physical stress. This result suggests that Rg1 may be a new candidate for treating stress related disorder.