HPLC/DAD에 의한 보중익기탕 중의 글리시리진산 정량분석법의 확립

Validation and Determination of Glycyrrhizic Acid as a Marker Substance in Bu-Zhong-Yi-Qi-Tang by HPLC/DAD

A high performance liquid chromatographic (HPLC) method for the determination of glycyrrhizic acid was developed for the quality control of traditional herbal medicinal preparation Bu-Zhong-Yi-Qi-Tang (BZYQT), which is wellknown herbal medicine used as tonic. RP-HPLC analysis was carried out using Capcell pak C18 MG column (5 μ, 150×4.6 mm) and a mobile phase consisting of acetonitrile and water containing 0.03% phosphoric acid (pH 2.46) at a flow rate of 1.0 ml/min. The optimum wavelength for the detection of the glycyrrhizic acid was found at 250 nm using diode-array UV/ VIS detector. The glycyrrhizic acid in BZYQT shows good linearity (r2>0.999) in the range of 15 μg/ml to 500 μg/ml. The limit of detection (LOD) was less than 5 ng and R.S.D for intra-day and inter-day reproducibility was less than 7%. The mean recovery of the glycyrrhizic acid was 97.3~113.0%. These results suggest that the developed HPLC method is simple and efficient, and could be contributed for the quality control of commercial BZYQT products.