원조포미바이러스 U5 LTR 말단의 보존적인 잔기의 돌연변이에 대한 인테그라제의 반응성

Reactivity of prototype Foamy Virus Integrase to the Mutants of the Highly Conserved Terminal Sequence of U5 LTR

The long terminal repeat (LTR) of retroiral DNA genome plays an important role in the integration process by providing substrate recognition site for viral integrase (IN). The dinucleotide CA near the 3'-end of the LTR termini is completely conserved among retroviruses. In order to study specificity of interaction between prototype foamy virus (PFV) IN and its U5 LTR DNA, the effect of mutagenesis of the CA sequence was investigated by studying reactivity of PFV IN to the mutant LTR substrates. Replacement of only the C or the A allowed 60 to 100% of the reactivity of the wild type LTR substrate. In addition, replacement of the C and the A showed 50 to 80% of the reactivity of the wild type LTR substrate, indication that PFV IN has less specificity on the conserved CA sequence when it is compared to the other retroviral INs. Therefore it is suggested that PFV IN is liss dependent on the conserved sequence of LTR termini for its enzymatic reaction.