안정동위원소 비율 및 테트라하이드로칸나비올 함량 분석을 통한 한국산 대마의 재배 환경 추론

Planting Condition of Korean Cannabis Derived from Stable Isotype Ratio & Tetrahydrocannabiol Contents

Stable isotope ratio of carbon and nitrogen (δ13C & δ15N), and ∆ 9-tetrahydrocannabinol (THC) contents were measured on 37 Korean cannabis and 10 commercial grade marijuana seized in Korea. Factors influencing on the measured values and their variations were investigated. δ13 value of cannabis is specified mainly by water availability. Korean cannabis showed relatively low δ13 values ranging -33.29~-27.01‰ (mean=-31.01‰), which reflect geographic conditions of Korea where is rainy, especially during summer. δ15N values, which reflect individual planting conditions, were relatively high up to -0.5~18.0‰ (mean=6.44‰). It reflects characteristics of Korean cannabis growing wild in forest or cultivated in fertile soil. Tetrahydrocannabinol is the major hallucinogenic compound of cannabis. Ethanol extracts of cannabis leaves were derivatized by N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the derivatives were analyzed by GC-MS in selected ion monitoring (SIM) mode. THC contents of Korean cannabis ranged 0.11~4.34% (mean=1.47%), which were relatively low compared with commercial grade marijuana.