랫드 심근세포유래 H9C2 세포주에서의 망간화합불의 산화적스트레스 유도작용

Induction of Oxidative Stress by Manganese Chloride in Cultured H9C2 Cells

Manganese is a naturally occuring element which is widespread in the enviroment. Also, manganese is an essential trace element and plays a key role in important biological reactions catalyzed by enzymes. However, exposure to high levels of manganese can cause toxicity in neurone and inhalation system, also damage in various tissues. We investigated the toxicity induced by manganese compound (MnCl< sub>2) in cultured rat cardiomyocytes. Treatment of manganese to cultured cardiomyocyte led to cell death, reactive oxygen species (ROS) increase, and cytosolic caspase-3 activation. The ROS increase was related with the decreased level of glutathione. Expressions of ROS related genes such as heme oxygenase-1, thioredoxin reductase, and NADH quinone oxidase were significantly induced in manganese treated cells. These results suggest that manganese induce oxidative stress and apoptosis in cardiomyocytes, and may be the one of risk factors to cause heart dysfunction in vivo.