Helicobacter pylori의 전사조절인자인 Fur 단백질과 Metronidazole 저항성

Transcriptional Regulatory Protein Fur of Helicobacter pylori and its Role in Metronidazole Resistance

To clarify effects of the structural changes if Fur protein on the resistance to metronidazole(MTz), the mutational analysis of structure and function of the protein in Helicobacter pylori (Hp) was undertaken. It was identified that some changes in Hp Fur protein resulted in increase og resistance to Mtz, and other changes resulted in decrease of resistance. Increase of Mtz resistance came from the enzyme's decreased ability of reducing prodrug Mtz to the form of bactericidal agent. Some sites that affects Mtz resistance (i) in Fur's N terminal extension, and (ii) in its central region, which links DNA binding and Fe-binding modules were identified. It was also found that the FLAG tag to Fur's C terminus also significantly impairs Fur function.