1-Furan-2-yl-3-pyridin-2-yl-propenone의 TNF-α 유도성 MCP-1과 IL-8의 발현 억제를 통한 장 상피세포 염증 억제효과

1-Furan-2-yl-3-Pyridine-2-yl-Propenone Inhibits TNF-α-induced Intestinal Inflammation via Suppression of MCP-1 and IL-8 Expressions in HT-29 Human Colon Epithelial Cells

Previously, we have shown that 1-furan-2-yl-3-pyridin-2-yl-propenone (FPP-3) has an anti-inflammatory activity in a rat paw-edema model. In the present study, we investigated an inhibitory effect of FPP-3 on the tumor necrosis factor (TNF)-α-induced inflammatory cytokine response in HT-29 human colon epithelial cells. Treatment with FPP-3 significantly prevented the TNF-α-induced attachment of leukocytes to HT-29 colon epithelial cells, which is one of the pathologic hallmarks in colon inflammation. The effect of FPP-3 was markedly superior than that of 5- aminosalicylic acid (5-ASA), a commonly used drug for the treatment of inflammatory bowel disease (IBD). The pretreatment with FPP-3 inhibited TNF-α-induced monocyte chemoattractant protein (MCP)-1, interleukin (IL)-8 mRNA expressions. In addition, FPP-3 significantly suppressed TNF-α-induced nuclear factor (NF)-κB transcription activity. These results demonstrate that FPP-3 modulates intestinal inflammation via suppressing the NF-κB dependent expressions of MCP-1 and IL-8, and suggest that FPP-3 may be a valuable agent for the treatment of IBD.