흰쥐 척수에서 지속성 진통물질 6-파라돌에 의한 아데노신의 유리 증가

Induction of Adenosine Release by 6-Paradol, a Long Lasting Analgesic, in Rat Spinal Cord

We previously demonstrated that 6-paradol, a compound structurally related to capsaicin, showed to produce prolonged analgesia in experimental animals. The effects of 6-paradol on the release of adenosine were investigated in the rat spinal cord synaptosomes by high performance liquid chromatography. In the presence of Ca++ adenosine was released from synaptosomes of rat spinal cord by 6-paradol and capsaicin in a dose dependent manner. Nifedifine, L-type voltage sensitive calcium channel blocker, was found to be ineffective in releasing adenosine by 10mcM 6-paradol. After exposure to 10mcM capsazepine, a novel capsaicin selective antagonist, the level of adenosine evoked by 10mcM 6-paradol was decreased by 75%, and that evoked by 10mcM capsaicin was blocked completely. These results suggest that the analgesic effect of 6-paradol might be mediated by the vanilloid (capsaicin) sensitive pathway, or the direct binding to the vanilloid receptor.