HPLC에 의한 목초액 중의 유기산, 페놀 및 벤조피렌의 분석법 개발

HPLC Analysis of Organic Acids, Phenol, and Benzopyrene in Wood Vinegar

The organic acids (formic acid, acetic acid and propionic acid), phenol and benzopyrene in wood vinegar were determine by HPLC. An AtlantisTMdC18 column with a acetonitrile : 0.1% phosphoric acid (5 : 95, organic acids), acetonitrile : water (10 : 90, phenol), 100% acetonitrile (benzopyrene) as a mobile phase was used. Retention time of acetic acid, formic acid, propionic acid, phenol and benzopyrene was 4.77, 3.73, 9.08, 30.97 and 6.10 min, respectively. The calibration curves of organic acids, phenol, benzopyrene were linear over the concentration range of 30~500, 60~1000, and 3~50 μg/ml with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of acetic acid, formic acid, propionic acid, phenol, and benzopyrene was 1.71 and 5.19, 1.11 and 3.35, 4.87 and 14.74, 6.45 and 19.55, 0.08 and 0.24 μg/ml, respectively. The coefficients of variation for intra- and inter-day assay were 0.21~4.14 and 0.07~1.19%, respectively and the precision was 95~115%. From this study, we suggest that HPLC is suitable method for the determination of organics in wood vinegar and could be applied to quality control of wood vinegar.