Bacillus lentimorbus B-6 균주로부터 생산된 α-Glucosidase 억제제의 생물 유화제로서의 특성

Emulsifying Character of α-Glucosidase Inhibitor Produced from Bacillus lentimorbus B-6

Bioemulsifiers are those chemicals which are produced from microorganisms but which have both hydrophilic and hydrophobic groups. α-Glucosidase inhibitor (α-GI) produced from Bacillus lentimorbus B-6 (B-6) showed bioemulsifying activity. But β-glucosidase inhibitor produced from B-6 didn't show emulsifying activity. α-GI was purified from supernatant of B-6 grown in minimal culture medium containing glucose and sodium glutamate by Sephadex G-100 column chromatography and isolated from β-GI by dialysis against water. Toluene was determined as the best substrate for emulsifying activity of α-GI. α-GI showed thermostability at 100oC for 15 min, high salt tolerance up to 32% NaCl and wide range of pH-stability at pH 4~10. Emulsifying character of α-GI can be useful for the liposome formation for the treatment of diabetes mellitus.