아데노바이러스를 이용한 성체 심실 근세포 이노시톨 1,4,5-삼인산 수용체 제 2 아형의 발현 억제

Knock-down of Type 2 Inositol 1,4,5-Trisphosphate Receptors using Adenovirus in Adult Ventricular Myocytes

Inositol 1,4,5-trisphosphate (IP3) receptor (IP3R)-mediated signaling pathway is involved in many cellular processes including fertilization, apoptosis and neuronal function. Although cardiac myocytes express the IP3R, its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. In the present study we constructed shRNA-expressing adenovirus to knock-down the type 2 IP3R (IP3R2), a major subtype in cardiac ventricular myocytes, and demonstrated that the virus successfully eliminated the expression and localization of the IP3R2. These results may provide a reliable tool for probing pathophysiological roles of the IP3R2 in isolated intact cardiac myocytes.