사염화탄소 유도 급성 간득성 모델에서 처자의 간 보호 효과

Protective Effect of Gardenia jasminoides Against Carbon Tetrachloride-Induced Acute Hepatotoxicity

Gardenia jasminoides is one of the most widely used herbal preparations for the treatment of liver disorders. This study evaluated the potential beneficial effect of G. jasminoides in a mouse model of carbon tetrachloride (CCI4)-induced liver injury. The mice were treated intraperitoneally with CCI4 (10 m모/kg). They received G. jasminoides (30, 100, 300 mg/kg) 48 h, 24 h and 2 h before and 6 h after administering CCI4 . The serum activities of aminotransferase and the hepatic level of malondialdehyde were significantly higher 24 h after the CCI4 treatment, while the concentration of reduced glutathione was lower. These changes were attenuated by G. jasminoides. CCI4 increased the level of circulating tumor necrosis factor-α (TNF-α) markedly, which was reduced by G. jasminoides. The levels of hepatic inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression were markedly higher after the CCI4 treatment. G. jasminoides diminished these alterations. CCI4 increased the level of TNF-a, iNOS and COX-2 mRNA expressions, and these increases were attenuated by G. jasminoides. These results suggest that G. jasminoides alleviates CCI4 -induced liver injury, and this protection is likely due to the reduced oxidative stress and the downregulation of proinflammatory mediators.