소나무 잎과 가지의 정유와 그 성분들의 항산화 및 항노화 작용

Anti-oxidant and Anti-aging Activities of Essential Oils of Pinus densiflora Needles and Twigs

To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular H2O2 and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is α-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is β-pinene (22.4%) in GC/MS assay. Other essential oils of PN were β-pinene, camphene, myrcene, limonene, terpinolene, α-terpineol, bornyl acetate, α-copaene, caryophellene and humulene and PT were α-pinene, camphene, phellandrene, limonene, terpinolene, α-terpineol, bornyl acetate, α-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular H2O2 and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and α-pinene have DPPH radical and superoxide scavenging activities, β-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular H2O2 and hydroxyl radical generations. And also phellandrene and β-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.