프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향

Effect of Prostaglandin F2αon the Production of Nitric Oxide in Cultured Ciliary Muscle Cells

Purpose: To investigate whether prostaglandin F2α (PGF2α) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. Methods: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2α (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, Nω- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. Results: PGF2α enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2α was not cytoprotective in serum-deprived and hypoxic conditions. Conclusions: The current results suggest that PGF2α potentiates NO production but is not cytoprotective in CM cells. This PGF2α-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow.