배양망막색소상피세포에서 젤라틴아제의 발현에 대한 산화스트레스의 영향

Effect of Oxidative Stress on the Expression of Gelatinases in Cultured RPE

Purpose: To evaluate the expression pattern of gelatinases in the human retinal pigment epithelial cell line ARPE-19 in response to oxidative stress. Methods: ARPE-19 cells were exposed to paraquat for 72 hours. The cells were assayed for the mRNA expression of gelatinase A and gelatinase B by reverse transcriptase polymerase chain reaction (RT-PCR). The conditioned media were assayed for the production of gelatinases protein by Western blotting. Also, the inhibitory effect of the antioxidants against oxidative stress to the cells was evaluated. Results: The gelatinase levels in the RPE cells increased under oxidative stress compaired with the control (p〈 0.05). Also, the levels decreased when the cells were incubated with the antioxidant N-acetylcysteine (NAC). Conclusions: Oxidative stress upregulates gelatinase expression in ARPE-19 cells.