트리암시놀론이 배양된 사람 섬유아세포의 생존과 산화질소 생성에 미치는 영향

Effect of Triamcinolone Acetonide on the Survival and Nitric Oxide Production in Cultured Human Tenon`s Capsule Fibroblasts

Purpose: To investigate the effect of triamcinolone acetonide (TA) on cell survival and nitric oxide (NO) production in cultured human Tenon`s capsule fibroblast (FB) cells. Methods: After exposed to TA, indomethacin, and dexamethasone for 2 days, cell survival and nitrite production of primarily cultured human FB cells were assessed with MTT and Griess assays, respectively. Results: Cell survival increased after exposure to TA at low concentrations but decreased at high concentrations. TA decreased the production of NO significantly (p<0.05). Exposure to indomethacin and dexamethasone revealed similar results. Conclusions: Both the biphasic effect on cell survival and the decreased production of NO induced by TA in FB cells could affect conjunctival wound healing