이마티니브 메실레이트에 의한 수정체상피세포의 섬유아세포로의 전환분화억제효과

Inhibitory Effects of Imatinib Mesylate on Transdifferentiation of Lens Epithelial Cells into Fibroblast

Purpose: To investigate whether Imatinib mesylate (IM) could inhibit the transdifferentiation of the lens epithelial cells (LECs) into fibroblasts using the capsular bag model. Methods: In the capsular bag model, LECs were cultured by exposure to IM at various concentrations for 3 min. The effect of IM was analysed by observing the covering area, numbers of α-SMA positive cell, and BrdU incorporated proliferating cells. The same analysis was performed in the culture of LECs with TGF-β. Results: The covering area was significantly decreased by the treatment of 30 ?M IM, and the positive cells for α-SMA and BrdU were also decreased by IM treatment in a dose dependent manner. In addition, increasing TGF-β concentration accelerated transdifferentiation, but suppressed the acceleration of the transdifferentiation induced by TGF-β. Conclusions: In the capsular bag model, IM effectively inhibited not only the transdifferentiation of LECs into fibroblasts but also the TGF-β induced acceleration of the transdifferentiation.