베체트 포도막염에서 CD8양성 T세포 표현형의 특성

Phenotypic Characteristics of CD8bright T Cells in Beh?et’s Uveitis

Purpose: To determine the phenotypic characteristics of peripheral CD8bright T cells. Methods: Forty patients with Beh?et’s disease (BD) with uveitis (active: 20; inactive: 20) and 20 healthy controls were recruited in this study. Phenotypic analysis of fresh PBMCs was performed using anti-CD8 mAb and anti-CD56 mAb in conjunction with a three- or four-color immunofluorescence tests for the expression levels of the following molecules: CD11b, CD27, CD45RA, CD45RO, CD62L, CD94, NKG2D, and HLA-DR. Results: CD27 and CD62L were down-regulated on peripheral CD8bright T cells in patients with active Beh?et’s uveitis in contrast to the up-regulation of CD11b and HLA-DR. These phenotypic natures were remarkable in the CD56+ subsets. CD45RAdimCD45RO- phenotypes were expanded in peripheral CD8brightCD56+ T cells according to the disease activity. Interestingly, in active Beh?et’s uveitis, CD94 was up-regulated on peripheral CD8brightCD56+ T cells in BD in contrast to the down-regulation of NKG2D compared with the normal controls. Conclusions: CD8brightCD56+ T cells are characterized by activated effector phenotypes with functional NK receptors and they may play immunopathogenic roles in Beh?et’s uveitis.