가토 각막에서 자가 윤부이식 시 성공을 위한 최소 윤부 양에 대한 연구

Minimal Requirement of Limbal Epithelium for Successful Limbal Cell Transplantation in Rabbit Corneas

Purpose: To investigate the minimal requirements of the limbal epithelium for successful limbal stem cell transplantation and the healing process. Methods: Nine rabbits were divided into 4, 6, and 8 clock-hour transplantation groups. Limbal autografts from the healthy fellow eye were transplanted to the iatrogenic damaged eye. The amniotic membrane served as a stem cell niche. Experimental corneas were evaluated by slit lamp examination and immunohistochemistry. Results: In the over 9 hours transplantation group, the healing process of the epithelium from the limbal stem cell was revealed and cornea-specific keratin k3, transcription factor p63, and connexin 43 were detected by immunohistochemistry. The normal corneal epithelium was regenerated after 60 days postoperatively in the fellow donor eye. Conclusions: Limbal cell transplantation of over 9 hours seems to be a safe and effective method in the treatment of severe ocular surface disorders. In addition, the donation of limbal epithelium for up to 8 hours did not affect the normal corneal regenerating capability.