수정체 상피세포에서 TGF-β에 의한 MMP-2와 MMP-14의 발현과 조절 기전

The Regulation of MMP-2 and -14 Expressions by TGF-β in Lens Epithelial Cells

Purpose: TGF-β is a key regulator of epithelial-mesenchymal transition. Among the TGF-β responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-β and examined the TGF-β signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) Methods: First we examined the expression of MMPs in rat lens explant culture treated with TGF-β and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. Results: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. Conclusions: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-β.