항알러지 점안약이 배양된 가토의 결막세포에 미치는 영향

The Effects of Anti-allergic Ophthalmic Agents on the Cultured Rabbit Conjunctival Cells

Purpose: To investigate the biological effects and cytotoxicity of anti-allergic ophthalmic agents on the cultured conjunctival cells of rabbit in vitro. Methods: Conjunctival cells of rabbit were exposed to anti-allergic ophthalmic agents. Azelan? (Taejoon, Korea), Zaditen? (Novartis, USA), Patanol? (Alcon, USA) at a concentration 10, 20 and 30% for a period of 30 minutes, 4, 12, and 24 hours respectively. Cell injury assay was performed using lactate dehydrogenase (LDH) leakage assay. We checked the composition, pH, osmolarity of three anti-allergic agents. Light and transmission electron microscopy were performed to compare the cellular damage of rabbit conjunctival cells under various culture treatments. Results: In cultured conjunctival cells of rabbit, the LDH titers increased up to 4 hours after exposure, maintained until 12 hours and then decreased 12 hours after exposed. Azelan? and Zaditen? showed a higher LDH titer and severe cellular damage of the conjunctival cells, compared with Patanol?. Of anti-allergic solutions, Azelan? and Zaditen? revealed markedly lower Na+, Cl- and pH levels than Patanol?. However, there was no difference in the concentration of preservative or osmolarity of the eye solution among the three anti-allergic agents. Conclusions: Patanol? caused markedly less damage to cultured rabbit conjunctival cells than Azelan? and Zaditen?. If these anti-allergic agents are clinically used for long periods of time, they may induce the cellular damage of conjunctival cells depending on the composition and pH of anti-allergic drugs.