섬유주세포에서 덱사메타존이 산화질소의 생성에 미치는 영향

Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells

Purpose: To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells. Methods: Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 μM sepiapterin, 100 μM ascorbic acid, and 10 μM methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively. Results: DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dosedependent manner. With co-exposure of DEX, ascorbic acid ified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production. Conclusions: DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow.