망막색소상피세포에서 포도당이 반응성산소종의 생성에 미치는 영향

Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells

Purpose: To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells. Methods: ARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 μM N-acetyl cysteine (NAC) and 100 μM L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively. Results: HG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant. Conclusions: HG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells. J Korean Ophthalmol Soc 2010;51(2):276-281