섬유주세포에서 Erythropoietin이 일산화질소의 생성에 미치는 영향

Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells

Purpose: To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR. Results: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity. Conclusions: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity. J Korean Ophthalmol Soc 2011;52(12):1514-1518