섬유아세포의 생존에 대한 베바시주맙과 마이토마이신 C의 효과 비교

Comparison of the Effects Between Bevacizumab and Mitomycin C on the Survival of Fibroblasts

Purpose: To compare the antiproliferative effects between bevacizumab, a monoclonal antibody against vascular endothelial growth factor (VEGF), and mitomycin C in human Tenon’s capsule fibroblasts. Methods: Primarily cultured human Tenon’s capsule fibroblasts were exposed to 0, 25, 50, 100, and 200 μg/ml bevacizumab and mitomycin C, and incubated for 5 days. Cellular survival and production of nitric oxide were assessed by MTT assay and Griess assay, respectively. Results: Bevacizumab showed antiproliferative effects only at high concentrations (200 μg/ml) and revealed much less effect on the cellular survival compared to mitomycin C. In addition, bevacizumab did not affect the production of nitric oxide. Conclusions: The antiproliferative effect of bevacizumab is much lower than mitomycin C in human Tenon’s capsule fibroblasts. J Korean Ophthalmol Soc 2011;52(3):345-349