혈청결핍 상태에서 크레아틴이 RGC-5세포의 생존에 미치는 영향

Effect of Creatine on the Survival of RGC-5 Cells under Serum Deprivation

Purpose: To evaluate the protective effect of creatine on the survival of retinal ganglion cells after serum deprivation. Methods: RGC-5 cells were exposed to 5 mM creatine with serum-free media for 4 days. Cellular survival and mitochondrial respiratory activity were measured with MTT assay and resazurin assay, respectively. Degree of apoptosis was evaluated with vital staining using acridine orange/Hoechest 33342 and flow cytometric analysis using annexin/PI, respectively. Results: Creatine increased cellular survival of RGC-5 cells significantly after serum deprivation. Additionally, creatine increased mitochondrial respiratory activity and inhibited apoptosis of RGC-5 cells. Conclusions: The energy precursor creatine increased survival of retinal ganglion cells after serum deprivation. Creatine could be relevant for the cytoprotection of retinal ganglion cells. J Korean Ophthalmol Soc 2011;52(5):618-623