건성안 환자의 눈물 내 케모카인 리간드 5의 농도 분석

Analysis of CCL5 Concentration in Tears of Dry Eye Patients

Purpose: To investigate the expression of CCL5/RANTES (regulated upon activation, normal T cell expressed and secreted) in the tears of dry eye patients. Methods: Forty patients with dry eye (15 Sj&ouml;gren’s and 25 non-Sj&ouml;gren’s syndrome patients) and ten control subjects were recruited for the present study. The concentration of RANTES in tears was measured using an enzyme-linked immunosorbent assay. The correlations between RANTES level, tear film and ocular surface parameters, including tear film break-up time, basal tear secretion, tear clearance rate, corneal sensation, keratoepitheliopathy, and conjunctival goblet cell density, were analyzed in patients with dry eye syndrome. Results: The concentrations of RANTES were 435.46 ± 104.45 pg/ml in Sj&ouml;gren’s syndrome patients, 257.42 ± 46.72 pg/ml in non-Sj&ouml;gren’s syndrome patients, and 97.53 ± 29.15 pg/ml in the control patients (p < 0.01). The levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy, and goblet cell density (p < 0.05). Conclusions: CCL5/RANTES level increases in the tears of dry eye patients and correlates with various tear film and ocular surface parameters. J Korean Ophthalmol Soc 2011;52(6):658-664