송아지 혈청 농도가 인체의 각막상피세포에 미치는 영향

Effects of Calf Serum on Human Corneal Epithelial Cells in Vitro

Purpose: To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro. Methods: The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1α, TGF-β1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy. Results: Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1α, TGF-β1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum. Conclusions: Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically. J Korean Ophthalmol Soc 2011;52(7):852-858