Agrobacteria, generally existing in the soil, is a kind of gram-negative bacteria, including Agrobacterium tumefaciems and Agrobaterium rhizogenes. It is necessary to use bacteriostatic agents to eliminate the Agrobacterium after co-cultivation in Agrobacterium-mediated method. Bacteriostatic agents probably changed the composition of culture media and affecte the regeneration of the tissues, which would reduce transformation efficiency. Therefore, it is very important to choose suitable antibiotics. This paper reviewed the application of bacteriostatic agents in plants genetic transformation to provide a reference for tea transgene. Timentin might be an alternative antibiotic in plant transformation, especially for tea.
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