Identification of Microbes on the Single Fungus Fermented Tea by Pyrosequencing

The high quality Chinese Fuzhuan brick-tea grows mainly a yellow colored fungus, Eurotium sp. which is called as a golden flowering tea. It is known that Eurotium species are the sexual states of Aspergillus species. A few studies reveal that extracts from microbial fermented teas contain natural antimicrobial components that have inhibitive effect on several food-borne pathogen and spoilage bacteria. In this study, golden flowering teas were manufactured using inoculated a single fungus, E. cristatum, fermented and identified the microbes in the fermented teas by means of pyrosequencing. The microbial fermented tea, which grows yellow colored fungi was manufactured through 5-step processing such as mother-tea preparation, inoculation, fermentation, ripeness and desiccation. The manufacturing processes were already applied to nation patent (2014-0010502). In order to grouping the microbes and survey some contamination with harmful microbes, the pyrosequencing was performed on 8-samples manufactured different conditions. The result have shown that the phylum was Ascomycota 99.8%, the class was Eurotiomycetes 99.8% among total 27 class, the order was Eurotiales 99.6~99.8% among total 41 order, the family was Trichocomaceae 99.6~99.8% among 48 family, and the genus was Aspergillus 94.3~97.9% among 63 genus. The8-samples had 100% similarity in above 5-classification except genus level, otherwise the genus level have shown 94.3~97.9% of Aspergillus and 1.8~5.3% of Penicillium. The microbial fermented tea was manufactured successfully by single fungus inoculation. The pyrosequencing results indicate that the genus Aspergillus is existed dominantly in the tea which is the asexual name of Eurotium.