Amino acids and alkaloids are the key components of the tea and play important roles in liquor taste. Up to now, methods have been established for estimating alkaloids or amino acids separately, however, method is still limited for simultaneous determination these two kinds of compounds since their physical and chemical characteristics are quite different. In this work, a LC-MS/MS method was established for rapidly simultaneous determination 21 amino acids and 4 alkaloids in tea liquor, without chemical derivation and complex mobile phase. The correlation coefficients of linear equation of all the compounds estimated by this LC-MS/MS method were higher than 0.99, the lowest detectable limit was 0.04μmol/ml at a signal-to-noise ratio of three, and the RSDs of accuracy were lower than 0.84% (for retention time) and 5.89% (for peak area), and the recovery rates ranged from 82.87% to 121.37%. This method could simultaneously quantify the amino acids and alkaloids in teas with rapid sample preparation and high sensitivity.
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