The Study of Pilot Process of Separation and Purification of Catechin Using Wood Fiber Resin

This paper studies the pilot production of tea polyphenol (TP) from membrane filtered tea extract using wood fiber resin. We extract, isolate and purify TP using ultrasonic wave extraction, ultrafiltration membrane and wood fiber resin column chromatography techniques. We then use high performance liquid chromatography (HPLC) to filter optimal parameters and get the final product using spray drying method. The results show that after above process the content of caffeine, EGCg and catechin in 40% eluent is 0.607%, 60.7%, 86.95% separately and the low caffeine high catechin procuct yield rate is 7.0%. While in 10% eluent the content of caffeine, EGCg and catechin is 26.6%, 16.5%, 23.7% separately, and the high caffeine low catechin product yield rate is 7.3%. So the total summed yield rate is 14.3%. The pilot process may provide reference for future green TP production.