발효 갈근탕과 쌍화탕의 혈소판 응집 억제 효과 연구

Anti-platelet Aggregation Study of Fermented Galgeun Tang and Fermented Ssanghwa Tang

This study was performed to evaluate enhanced effect of fermented Galgeun tang (GGT) and Ssanghwa tang (SHT) on the anti-platelet aggregation. Platelet aggregation assay was performed In vitro using human platelet rich plasma(PRP) and In vivo using SD-rat plasma by platelet aggregometer. Pharmacodynamic parameters, Emax and EC50, were calculated using Winnolin. SD-rats administered 1 g/kg of oriental medicine every 12 hr for 8 days. Platelet aggregation was measured by optical method with collagen inducer (4 μg/ml). In In vitro anti-platelet study, EC50 of GGT-A was lower than that of GGT-con about 79.13 μg/ml. And EC50 of SHT-A and SHT-B was lower than that of SHT-con about 122.73 and 110.15 μg/ml, respectively. It is assumed that fermented GGT and SHT were more effective than original medicine. In multiple administration study, anti-platelet effect was significantly increased both GGT and SHT. Fermented GGT and SHT were more effective than original herbal medicine on anti-platelet aggregation.