3T3-L1 세포에서 소맥엽 에탄올추출물의 지질생성 억제효과

Inhibitory Effect of Triticum aestivum Ethanol Extract on Lipid Accumulation in 3T3-L1 Preadipocytes

Non-alcoholic fatty liver disease is known to be frequently associated with obesity and type 2 diabetes. We examined the effects of EtOH extracts from Triticum aestivum on lipid accumulation during the differentiation of 3T3-L1 preadipocytes to screening the candidate materials in preventing non-alcoholic fatty liver disease. The lipid level in adipocytes was determined by Oil Red O staining. The treatment of 50% ethanol, but not water and 100% ethanol extracts, from Triticum aestivum at concentration of 0.5 mg/ml inhibited lipid accumulation in 3T3-L1 cells, revealing no cell toxicity. Thus, the fractions of CH2Cl2, EtOAc and BuOH were separated from 50% EtOH extract to characterize anti-adipogenic effect. The CH2Cl2 fraction at concentration of 50 μg/ml effectively inhibited the lipid accumulation in the adipocytes compared to those of EtOAc and BuOH at concentration of 50 μg/ml. The intracellular triglyceride accumulation also was significantly reduced by treatment of CH2Cl2 fraction in concentration-dependent manner. Western blot analysis showed that the CH2Cl2 fraction attenuated the intracelluar level of fatty acid synthase(FAS) accompanied by attenuated expression of Peroxidase proliferator-activated receptor γ (PPARγ) adipogenic transcription factor. These results suggest that CH2Cl2 fraction from 50% EtOH extract of Triticum aestivum may has the potent anti-adipogenic effects by inhibiting the transactivation of PPARγ.