HPLC On-line ABTS+ Screening을 이용한 왕대(Phyllostachys bambusoides) 잎으로부터 Homoorientin의 확인

Rapid Identification of Homoorientin from Phyllostachys bambusoides Leaves by HPLC On-line ABTS+ Screening Method

To determination of antioxidant substance homoorientin, from Phyllostachys bambusoides leaves, the ultrasonic extraction and HPLC on-line ABTS+ screening method were empolyed. Also, the various experimental variables such as the frequency and time of ultrasonic system were investigated and homoorientin was extracted efficiently at the low frequency 35 kHz and the extraction time 60 min. The values were positive peak 1574.71 (relative area, 23.67%) and negative peak 6924.34 (relative area, 1.23%), respectively. This HPLC on-line ABTS+ screening method was rapid and efficient to search for antioxidants from natural products. These results will provide a database for investigating the constituents of natural products and the resources of pharmaceutical and cosmetic products.