전단 자극에 의한 심방 근세포 칼슘 웨이브의 발생 : Phospholipase C-이노시톨 1,4,5-삼인산 수용체 신호전달의 역할

Activation of a Ca2+ wave by Shear Stress in Atrial Myocytes : Role of Phospholipase C-inositol 1,4,5-Trisphosphate Receptor Signaling

Cardiac myocytes are subjected to fluid shear stress during each contraction and relaxation. Under pathological conditions, such as valve disease, heart failure or hypertension, shear stress in cardiac chamber increases due to high blood volume and pressure. The shear stress induces proarrhythmic longitudinal global Ca2+ waves in atrial myocytes. In the present study, we further explored underlying cellular mechanism for the shear stress-induced longitudinal global Ca2+ wave in isolated rat atrial myocytes. A shear stress of ~16 dyn/cm2 was applied onto entire single myocyte using pressurized fluid puffing. Confocal Ca2+ imaging was performed to measure local and global Ca2+ signals. Shear stress elicited longitudinally propagating global Ca2+ wave (~80 μm/s). The occurrence of shear stress-induced atrial Ca2+ wave was eliminated by the inhibition of ryanodine receptors (RyRs) or inositol 1,4,5-trisphosphate receptors (IP3Rs). In addition, pretreatment of phospholipase C (PLC) inhibitor U73122, but not its inactive analogue U73343, abolished the generation of longitudinal Ca2+ wave under shear stress. Our data suggest that shear-induced longitudinal Ca2+ wave may be induced by Ca2+-induced Ca2+ release through the RyRs which is triggered by PLC-IP3R signaling in atrial myocytes.