Epigallocatechin Gallate (EGCG)의 포름알데히드에 의한 폐 대식세포 사멸 차단 효과

The Protective Effect of Epigallocatechin Gallate (EGCG) against Formaldehyde-induced Cell Death in Alveolar Macrophage

The consumption of green tea (GT) is linked to a decrease in the onset of diverse diseases in humans. Epigallocatechin-3-gallate (EGCG) is the major catechin of green tea. Formaldehyde (FA) is an important irritant compound in pesticides to induce asthma and allergy in the respiratory system. Alveolar macrophages are also a pivotal cell type in the immune response of the respiratory system. On the other hand, the effects of EGCG on FA-induced cell death has not been elucidated. Therefore, this study examined the preventive effects of EGCG against FA-induced cell death in Raw 264.7 cells, an alveolar macrophage cell line. In the present study, FA induced a decrease in the cell viability and GSH contents, and stimulated lipid peroxide [LPO] formation. A treatment with green tea extracts (GTE) and EGCG (> 1 μM) prevented FA-induced cell death, lipid peroxide formation, and GSH contents in Raw 264.7 cells. The treatment with GTE and EGCG also prevented the FA-induced increase of cleaved caspase-3 and Bax, pro-apoptotic proteins, and a decrease in Bcl-2, an anti-apoptotic protein in Raw 264.7 cells. Hence, it is expected that EGCG from green tea offers protection against FA-induced cell death and its main action is mediated by a decrease in oxidative stress. In conclusion, green tea extracts prevented FA-induced cell death by decreasing the levels of oxidative stress in Raw 264.7 cells.