β-Sitosterol이 HCT116 대장암세포의 콜로니형성 및 부착성에 미치는 효과

Effects of β-sitosterol on colony formation and adhesion in HCT116 human colon cancer cells

β-Sitosterol is the most abundant phytosterol present ubiquitously in plants. The aim of the current study was to investigate the inhibitory effects of β-sitosterol on colony formation and adhesion in HCT116 human colon cancer cells. β-Sitosterol significantly inhibited cell growth (to 50-89% of the control by the concentrations of 25-100 μM; p<0.01) and effectively changed nuclear morphology (by the concentration of 50 μM) in HCT116 cells. These results confirm previous findings on the growth-inhibitory and apoptosis-inducing activities of β-sitosterol in different cancer cell lines. Treatment with β-sitosterol resulted in significant inhibition of both colony formation (to 29% of the control by the concentration of 50 μM) and adhesion (to 44-54% of the control by the concentration of 25-100 μM) in HCT116 cells. These results suggest that β -sitosterol might modulate the self-renewal capacity of colon cancer stem cells as well as invasion and metastasis during colon carcinogenesis. More in-depth studies are needed in order to verify such actions and to elucidate underlying mechanisms.