B16 흑색종세포에서 아라키돈산에 의한 멜라닌 합성 촉진 효과에 미치는 칼륨-염소이온-수송체의 역할

Role of K⁺₋Cl⁻₋cotranspoter in the Arachidonic Acid-induced Stimulation of Melanin Synthesis in B16 Melanoma Cells

The effect of arachidonic acid (AA) on melanogenesis was investigated in B16 melanoma cells. AA induced melanogenesis and K⁺ efflux in a dose-dependent manner, which was significantly inhibited by DIOA, a specific inhibitor of K⁺₋Cl⁻₋cotransporter (KCC). These effects of AA were not altered by inhibitors of cyclooxygenase and lipoxygenase. Treatment with ETYA, a non-metabolizable analog of AA induced the same effects as AA did. Taken together, these results suggest that AA itself induced melanogenesis, and that KCC may mediate the effects of AA.