서울특별시 종로구 대중목욕탕의 수질 중 미샘물 오염도 조사 연구

Investigation of Microbial Contamination of Public Bath in Jongno-gu, Seoul

The aim of this study is to investigate microbial sanitary condition of public baths in Seoul, Korea. A total of 28 water samples werε collected from 14 different public baths and sudatoriums. The preva1ence of fecal indicator microorganisms such as tota1 coliform, feca1 coliform, and Escheri‘chia coli was characterized. 1n addition, bacteria in water was membrane filtered by 0.45 um nitrocellulose membrane, and the filter was analyzed by both cultivation dPCR amplification of ptial 16S rRNA gεne. The levels of chlorine were measured for each of water snples. More than 40% of 14 collected water samples, the concentrations of total coliform bacteria exceeded the water qua1ity for bath water guideline. There was no significant correlation between chlorine rεsidue and the presence of total coliform. Various microorganisms including pathogenic microorganisms were identitìed from cultivationd subsequent analysis of 16s rRNA gene sequencεs. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing hea1th risk associated with public batlouses.