보이차(Pu-erh tea)의 HaCaT 세포보호 및 HCT116 세포사멸 효과

Effect of Pu-erh tea on UVB-induced oxidative damage in human HaCaT and cytotoxicity in HCT116 human colon cancer cells

The present study was performed to investigate the biological activity of fermented Pu-erh (FP) and raw Pu-erh (RP) tea extractives. In this study, we examined the antioxidants with the DPPH and ABTS assays and conducted an apoptotic analysis with the MTT assay and western blotting experiments. The total phenolic contents in FP and RP were 38.59 ± 15.23 and 36.83 ± 6.60 mg GAE/g dry weight, respectively. The effects of FP and RP on the apoptotic pathway were also investigated. It was found that the levels of Bcl-2 decreased, whereas those of Bax and cleaved caspase-9, caspase-3 and PARP increased in a dose-dependent manner. In addition, we investigated the protective effects of FP and RP against ultraviolet B-induced oxidative damage in human keratinocytes (HaCaT cells). To induce oxidative stress in the HaCaT cells, they were irradiated with UVB at 30 mJ/㎠. FP and RP was found to be reduced phosphorylation of IkBα and IKKα/β, block nuclear factor-kB p65 nuclear translocation, and strongly suppress the pro-inflammatory mediators.