백서 배자의 악안면 영역 발생에서 TGF-β2의 발현에 관한 면역조직화학적 연구

THE IMMUNOHISTOCHEMICAL STUDY ON THE EXPRESSION OF TGF-β2 IN THE MAXILLOFACIAL DEVELOPMENT OF A RAT EMBRYO

TGF-β was identified as a homodimeric peptide with a molecular mass of 25,000 D, and is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell type. Many cells synthesize TGF-β and essentially all of them have specific receptors for this peptide. TGF-β regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. At present there is relatively little information about the intrinsic physiologic role of TGF-β in vivo. Experimental study was performed to observe the expression of TGF-β2 in the craniomaxillofacial development of a rat embryo. Four male rats each weighing about 250g and twelve female rats each weighing about 200g were used, and were induced to pregnancy. The embryos were serially sacrificed on the 13, 15, 18day after coitus. The embryos were serially sectioned by 4㎛ and stained with H&E and immunohistochemical stain with TGF-β2 and the histological examinations were carried out under the light microscope. After examination and comparision of all specimens, the results of this study were as follows : 1. In a 15, 18d embryos, low grade stain of TGF-β2 is found in the dental papillar of developing incisor, and around the Meckle s cartilage and the other cartilage TGF-β2 is positive. In the formation of palate, TGF-β is highly positive. 2. In a development of teeth in 15, 18d embryos, TGF-β2 is seen in the enameloblast derived from oral epithelium and the mesenchymal primordium of the pulp. 3. In a epithelium of 13, 15. 18d embryos, TGF-β2 is seen at a high levels. 4. In a 13, 15, 18d embryos, TGF-β2 positivity of nasal cavity epithelium is maybe related to the sensory organ epithelium. In a 15, 18d embryos, the lens of the eye derived from the surface embryonic ectoderm is TGF-β2 positive. From above results, staining of TGF-β is closely associated with tissues derived from neural crest mesenchyme and tissues of sensory organ derived from ectoderm. Marked staining is also seen when remodeling of mesenchyme or mesoderm occurs, as during formation of palate and cartilage.