DMBA 유도 hamster 협낭 발암과정에서의 capsaicin의 효과

THE EFFECT OF CAPSAICIN UPON DMBA INDUCED CANCER OF THE BUCCAL POUCH IN HAMSTERS

Cancer chemoprevention can be defined as prevention or intervention of cancer by the administration of one or more chemical entities. either as individual drugs or as naturally occurring constituents of the diet. The name capsaicin(trans-8-methyl-N-vanillyl-6-nonenamide) was given by Thresh in 1848, and capsaicin is a primary pungent and irritating principle present in red peppers which are widely used as spices in korean food. The inhibitory effect of capsaicin on stomach or skin cancers had been reported in lots of animal studies, but there were few reports in oral cancer carcinogenesis. This study is aimed to see the effect of capsaicin upon DMBA induced cancer of the buccal pouch in hamsters. In this study, 87 Golden Syrian hamsters, weighing about 80-90g, were used and divided into 5 groups : normal group(n=2)-untreated ; control group(n=15)-DMBA painted ; experimental group Ⅰ(n=23)-capsaicin(10/μmol/2㎖) and DMBA painted ; experimental group Ⅱ(n=23)-capsaicin administered and DMBA painted ; experimental group Ⅲ(n=24)-capsaicin administered and painted, DMBA painted. According to groups, the animals were sacrificed at 5, 8, 11, 14, 17 week. Microscopic examination was done and BrdUrd labeling index was calculated. The results are as follows ; 1. According to gross examination, the leukoplakia at 5 week and the papilloma at 8 week were shown in the experimental group Ⅰ, Ⅲ. Those feature were appeared rapider than those of the control and experimental group Ⅱ. 2. According to gross examination, the exophytic tumorous lesions were shown in the control and all of the experimental groups from 14week, but the features of the control group were severer than those of the experimental groups 3. According to histopathologic features, the papilloma was shown in all of the experimental group except the control group at 8 week 4. Histologically, the features of the control group were severer than those of the experimental groups from 14 week and there were similiar features among the experimental group Ⅰ, Ⅱ, Ⅲ at 17 week 5. At 8 week, BrdUrd labeling index of experimental group Ⅰ, Ⅲ were higher than that of control group and it was statistically significant(p＜0.05) 6. The BrdUrd labeling index of experimental group Ⅲ from 11 week, experimental group Ⅰ from 14 week, experimental group Ⅱ from 17 week were lower than that of the control group respectively and it was statistically significant(p＜0.05) 7. In the distribution of the BrdUrd labeled cells, BrdUrd was more significant in the basal and parabasal cells in dysplatic changes, but in carcinoma in situ, BrdUrd showed a irregular arrangement throughout the whole thickness of the tumor epithelium According to the above results, the irritating property of the capsaicin accelerated the carcinogenesis in early phase, but the inhibitory effect was shown from carcinoma in situ phase. So the chemopreventive effect of the capsaicin which is a major ingredient of red pepper can be expected. However, the further studies including the proper amount and method of capsaicin administration for preventive effect of oral cancer, should be followed.