백서에서 냉동건조골, 표면탈회골, 탈회골분말을 골형성 단백질과 병용시 골유도 과정에 대한 비교 연구

COMPARISON OF BONE INDUCING PROCESS OF PORCINE BONE MATRIX-DERIVED BMP COMBINED WITH THE FOLLOWING, FREEZE-DRIED ALLOGENEIC BONE, SURFACE DEMINERALIZED ALLOGENEIC BONE, AND DEMINERALIZED ALLOGENEIC BONE POWDER IN RATS.

The purpose of this study is to compare the osteogenic activity of pBMP combined with the following, freeze-dried allogeneic bone, surface demineralized allogeneic bone, and demineralized allogeneic bone powder in rats. So, authors made partial purification of the pBMP. Using rat calvaria, an 8㎜ size in diameter bony defect was grafted using pBMP-ibm in the experimental group A as the control group, freezed dried allogeneic bone graft plus pBMP-ibm in the experimental group B, surface demineralized allogeneic bone graft plus pBMP-ibm in the experimental group C, and demineralized allogeneic bone powder plus pBMP-ibm in the experimental group D, respectively. The rats were sacrificed at the week of 1st, 2nd, 4th, 8th after implantation and examined grossly, biochemically, biomechanically, microscopically. The results were as follows 1. Areas of Implanted defect acquired osseous union at post op 4th week. 2. According to the result of hardness test, the experimental group B was most rigid than any other experimental groups at post op. 4th week. And the experimental group B, C and D were more rigid than the experimental group A at post op. 8th week. 3. As time goes on, calcium content of implanted area has increased gradually in all experimental groups except the experimental group B. 4. In the early stage of grafting, the new cartilage formation & new bone formation were more active in the experimental group B, C and D than in the experimental group A, but in the late stage of grafting, there was no significant difference in the new bone formation among the experimental groups. 5. New cartilage and new bone formation occurred as folIows, first in the experimental group D, then the experimental group C, the experimental group B, the experimental group A, respectively.